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Addgene

FUGW-VGLUT2-pHluorin
(Plasmid #207469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VGLUT2
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Slc17a6 (a.k.a. Dnpi, Vglut2)
  • Tag / Fusion Protein
    • pHluorin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-VGLUT2-pHluorin was a gift from Robert Edwards (Addgene plasmid # 207469 ; http://n2t.net/addgene:207469 ; RRID:Addgene_207469)

  • For your References section:

    Synaptic Vesicle Recycling Pathway Determines Neurotransmitter Content and Release Properties. Silm K, Yang J, Marcott PF, Asensio CS, Eriksen J, Guthrie DA, Newman AH, Ford CP, Edwards RH. Neuron. 2019 May 22;102(4):786-800.e5. doi: 10.1016/j.neuron.2019.03.031. Epub 2019 Apr 16. 10.1016/j.neuron.2019.03.031 PubMed 31003725
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