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Addgene

FUGW-VGAT-pHluorin
(Plasmid #207467)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    VGAT
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Slc6a12 (a.k.a. BGT1, Gat1, RNU28927, VGAT)
  • Tag / Fusion Protein
    • pHluorin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.08.07.552338 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-VGAT-pHluorin was a gift from Robert Edwards (Addgene plasmid # 207467 ; http://n2t.net/addgene:207467 ; RRID:Addgene_207467)

  • For your References section:

    Adaptor Protein-3 Produces Synaptic Vesicles that Release Phasic Dopamine. Jain S, Yee AG, Maas J, Gierok S, Xu H, Stansil J, Eriksen J, Nelson A, Silm K, Ford CP, Edwards RH. bioRxiv 2023 10.1101/2023.08.07.552338
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