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Addgene

pET19-TdTc180
(Plasmid #207462)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207462 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TdTc180
  • Species
    M. musculus (mouse)
  • Mutation
    Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser
  • Entrez Gene
    Dntt (a.k.a. Tdt)
  • Tag / Fusion Protein
    • 10xHis (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET19-TdTc180 was a gift from Jay Keasling (Addgene plasmid # 207462 ; http://n2t.net/addgene:207462 ; RRID:Addgene_207462)

  • For your References section:

    De novo DNA synthesis using polymerase-nucleotide conjugates. Palluk S, Arlow DH, de Rond T, Barthel S, Kang JS, Bector R, Baghdassarian HM, Truong AN, Kim PW, Singh AK, Hillson NJ, Keasling JD. Nat Biotechnol. 2018 Aug;36(7):645-650. doi: 10.1038/nbt.4173. Epub 2018 Jun 18. 10.1038/nbt.4173 PubMed 29912208
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