IVT-nCas9(H840A)
(Plasmid #207455)

• Purpose: in vitro transcription of mRNA
• Depositing Lab: Erik Sontheimer
• Publication: Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.1038/s41587-023-01947-w.

Backbone

• Vector backbone: IVT (In vitro Transcription) mRNA Production
• Backbone manufacturer: IVT (In vitro Transcription) mRNA Production
• Modifications to backbone: IVT (In vitro Transcription) mRNA Production
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H840A

Resource Information

• Supplemental Documents:
  • H840A_BNT_mRNA (2).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has been found to exist as a dimer. Plasmid dimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.