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Addgene

pPI896
(Plasmid #207453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPI465
  • Vector type
    Unspecified
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Nap1
  • Species
    Dictyostelium discoideum
  • Tag / Fusion Protein
    • mNeonGreen (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPI896 was a gift from Rob Kay (Addgene plasmid # 207453 ; http://n2t.net/addgene:207453 ; RRID:Addgene_207453)

  • For your References section:

    Formation and closure of macropinocytic cups in Dictyostelium. Lutton JE, Coker HLE, Paschke P, Munn CJ, King JS, Bretschneider T, Kay RR. Curr Biol. 2023 Aug 7;33(15):3083-3096.e6. doi: 10.1016/j.cub.2023.06.017. Epub 2023 Jun 27. 10.1016/j.cub.2023.06.017 PubMed 37379843
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