pPI896
(Plasmid
#207453)
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PurposeREMI integrated Expression for mNeonGreen-Nap1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPI465
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Vector typeUnspecified
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNap1
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SpeciesDictyostelium discoideum
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Tag
/ Fusion Protein
- mNeonGreen (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPI896 was a gift from Rob Kay (Addgene plasmid # 207453 ; http://n2t.net/addgene:207453 ; RRID:Addgene_207453) -
For your References section:
Formation and closure of macropinocytic cups in Dictyostelium. Lutton JE, Coker HLE, Paschke P, Munn CJ, King JS, Bretschneider T, Kay RR. Curr Biol. 2023 Aug 7;33(15):3083-3096.e6. doi: 10.1016/j.cub.2023.06.017. Epub 2023 Jun 27. 10.1016/j.cub.2023.06.017 PubMed 37379843
