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Addgene

hIRE1a K599A
(Plasmid #20745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV/TO Puro DEST (670-1)
  • Backbone manufacturer
    Eric Campeau (Addgene plasmid 17293)
  • Backbone size w/o insert (bp) 9594
  • Vector type
    Mammalian Expression, Lentiviral ; Destination vector
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2934
  • Mutation
    K599A, dominant negative kinase mutant
  • Entrez Gene
    ERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site Gateway (unknown if destroyed)
  • 3′ cloning site Gateway (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer MSCV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IRE1a cDNA was cut out of pENTR1A with EcoRI and XhoI and ligated into pLenti CMV/TO Puro DEST through Gateway cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hIRE1a K599A was a gift from Fumihiko Urano (Addgene plasmid # 20745 ; http://n2t.net/addgene:20745 ; RRID:Addgene_20745)

  • For your References section:

    The role of IRE1alpha in the degradation of insulin mRNA in pancreatic beta-cells. Lipson KL, Ghosh R, Urano F. PLoS ONE. 2008 . 3(2):e1648. 10.1371/journal.pone.0001648 PubMed 18286202
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