pDM1485
(Plasmid
#207446)
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PurposeExtrachromosomal expression of GFP-MhcA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDM1207
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Vector typeUnspecified
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemhcA
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Alt nameMyosin Heavy Chain A
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SpeciesDictyostelium discoideum
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note Aug 2023: The plasmid details in the Materials and Methods in Lutton et al Curr Biol. 2023 are currently incorrect. The details on this page are correct.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDM1485 was a gift from Rob Kay (Addgene plasmid # 207446 ; http://n2t.net/addgene:207446 ; RRID:Addgene_207446) -
For your References section:
Formation and closure of macropinocytic cups in Dictyostelium. Lutton JE, Coker HLE, Paschke P, Munn CJ, King JS, Bretschneider T, Kay RR. Curr Biol. 2023 Aug 7;33(15):3083-3096.e6. doi: 10.1016/j.cub.2023.06.017. Epub 2023 Jun 27. 10.1016/j.cub.2023.06.017 PubMed 37379843
