pPI560
(Plasmid
#207444)
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Purpose6xHis-SS-TEV-ErkB-Y178A bacterial expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSET
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameErkB
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SpeciesDictyostelium discoideum
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MutationY178A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPI560 was a gift from Rob Kay (Addgene plasmid # 207444 ; http://n2t.net/addgene:207444 ; RRID:Addgene_207444) -
For your References section:
The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module. Nichols JME, Paschke P, Peak-Chew S, Williams TD, Tweedy L, Skehel M, Stephens E, Chubb JR, Kay RR. Dev Cell. 2019 Feb 25;48(4):491-505.e9. doi: 10.1016/j.devcel.2018.12.001. Epub 2019 Jan 3. 10.1016/j.devcel.2018.12.001 PubMed 30612939