Skip to main content
Addgene

PGEX3X-GST-PTEN
(Plasmid #20743)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20743 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX-3X
  • Backbone size w/o insert (bp) 4952
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTEN
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_000314
  • Entrez Gene
    PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PGEX3X-GST-PTEN was a gift from Alonzo Ross (Addgene plasmid # 20743 ; http://n2t.net/addgene:20743 ; RRID:Addgene_20743)

  • For your References section:

    Allosteric activation of PTEN phosphatase by phosphatidylinositol 4,5-bisphosphate. Campbell RB, Liu F, Ross AH. J Biol Chem. 2003 Sep 5. 278(36):33617-20. 10.1074/jbc.C300296200 PubMed 12857747
Related items:
Commonly requested with: