pDM767
(Plasmid #207425)

• Purpose: Extrachromosomal expression HSPC300-GFP and PH-CRAC-mRFPmars
• Depositing Lab: Rob Kay
• Publication: Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.7554/eLife.20085.

Backbone

• Vector backbone: pDM459
• Vector type: Unspecified
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: HSPC300
• Species: Dictyostelium discoideum
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Gene/Insert 2

• Gene/Insert name: CRAC
• Alt name: DagA
• Species: Dictyostelium discoideum
• Mutation: aa 1–126 (PH domain) only
• Tag / Fusion Protein:
  • mRFPmars (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry