mAIRN CD
(Plasmid #207412)

• Purpose: Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
• Depositing Lab: Karlene Cimprich
• Publication: Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. doi: 10.1016/j.cell.2017.07.043.

Backbone

• Vector backbone: pCEP4 Mammalian Expression Vector
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 10400
• Total vector size (bp): 10822
• Modifications to backbone: 1) Replacement of pCMV with tight-Tet-ON promoter; 2) Switching orientation of tight-Tet-ON promoter by SalI restriction enzyme digestion and religation; 3) Cloning of ECFP or mAIRN sequence downstream of tight-Tet-ON promoter
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mAIRN
• Species: M. musculus (mouse)
• Insert Size (bp): 1401

Resource Information

• Supplemental Documents:
  • pSH37.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry