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Addgene

mAIRN CD
(Plasmid #207412)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207412 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCEP4 Mammalian Expression Vector
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 10400
  • Total vector size (bp) 10822
  • Modifications to backbone
    1) Replacement of pCMV with tight-Tet-ON promoter; 2) Switching orientation of tight-Tet-ON promoter by SalI restriction enzyme digestion and religation; 3) Cloning of ECFP or mAIRN sequence downstream of tight-Tet-ON promoter
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mAIRN
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1401

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mAIRN CD was a gift from Karlene Cimprich (Addgene plasmid # 207412 ; http://n2t.net/addgene:207412 ; RRID:Addgene_207412)
  • For your References section:

    Transcription-Replication Conflict Orientation Modulates R-Loop Levels and Activates Distinct DNA Damage Responses. Hamperl S, Bocek MJ, Saldivar JC, Swigut T, Cimprich KA. Cell. 2017 Aug 10;170(4):774-786.e19. doi: 10.1016/j.cell.2017.07.043. 10.1016/j.cell.2017.07.043 PubMed 28802045