mAIRN HO
(Plasmid #207411)

• Purpose: Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
• Depositing Lab: Karlene Cimprich
• Publication: Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. doi: 10.1016/j.cell.2017.07.043.

Backbone

• Vector backbone: pCEP4 Mammalian Expression Vector
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 10400
• Total vector size (bp): 10822
• Modifications to backbone: 1) Replacement of pCMV with tight-Tet-ON promoter; 2) Cloning of ECFP or mAIRN sequence downstream of tight-Tet-ON promoter
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mAIRN
• Species: M. musculus (mouse)
• Insert Size (bp): 1401

Resource Information

• Supplemental Documents:
  • pSH36.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The ~500bp LEXA cluster sequence is in the opposite orientation in the plasmid compared to the depositor's sequence. This region is located far away from any critical regions in the plasmid, and is not expected to impact plasmid function.