PET30B-PTEN
(Plasmid #20741)

• Depositing Lab: Alonzo Ross
• Publication: Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71.

Backbone

• Vector backbone: PET30b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PTEN
• Species: H. sapiens (human)
• GenBank ID: NM_000314
• Entrez Gene: PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
• Tag / Fusion Protein:
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7 terminator

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The cDNA encoding full-length (1-403) human PTEN was cloned into the NdeI and XhoI sites of the pET30b vector, thereby introducing a six histidine tag at the C-terminus.