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pWZL-Blast FAP A657S
(Plasmid #207403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWZL
  • Vector type
    Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fibroblast Activation Protein Alpha
  • Alt name
    FAPalpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2287
  • Mutation
    changed Alanine 657 to Serine. It's an endopeptidase-dead mutant
  • GenBank ID
    NM_004460.5
  • Entrez Gene
    FAP (a.k.a. DPPIV, FAPA, FAPalpha, SIMP)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL-Blast FAP A657S was a gift from Jing Yang (Addgene plasmid # 207403 ; http://n2t.net/addgene:207403 ; RRID:Addgene_207403)

  • For your References section:

    Fibroblast activation protein drives tumor metastasis via a protease-independent role in invadopodia stabilization. Bukhari M, Patel N, Fontana R, Santiago-Medina M, Jiang Y, Li D, Pestonjamasp K, Christiansen VJ, Jackson KW, McKee PA, Yang J. Cell Rep. 2023 Oct 31;42(10):113302. doi: 10.1016/j.celrep.2023.113302. Epub 2023 Oct 19. 10.1016/j.celrep.2023.113302 PubMed 37862167
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