KW78_HART_ODC1_PuroR
(Plasmid #207401)

• Purpose: Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR - PuroR
• Depositing Lab: Stephen Floor
• Publication: Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 10.1261/rna.079837.123.

Backbone

• Vector backbone: BdLV
• Backbone manufacturer: Luigi Naldini , PMID 15619618
• Modifications to backbone: The constitutive bidirectional promoter in bidirectional lentiviral vector (BdLV) was used to express eGFP under direction of a control 5'UTR and mCherry under control of the ODC1 5'UTR.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP
• Species: Synthetic
• Insert Size (bp): 726
• GenBank ID: 8382257
• Promoter: mini CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pkw78_bd_puro_eg_min_cp_odc1_mcd4.gb
• A portion of this plasmid was derived from a plasmid made by: Backbone is from PMID 15619618

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.14.557805 for bioRxiv preprint.