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KW57_HART_RAC1
(Plasmid #207399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207399 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BdLV
  • Backbone manufacturer
    Luigi Naldini , PMID 15619618
  • Modifications to backbone
    The constitutive bidirectional promoter in bidirectional lentiviral vector (BdLV) was used to express eGFP under direction of a control 5'UTR and mCherry under control of the RAC1 5'UTR.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    726
  • GenBank ID
    8382257

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.14.557805 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KW57_HART_RAC1 was a gift from Stephen Floor (Addgene plasmid # 207399 ; http://n2t.net/addgene:207399 ; RRID:Addgene_207399)

  • For your References section:

    A novel reporter for helicase activity in translation uncovers DDX3X interactions. Wilkins KC, Schroeder T, Gu S, Revalde JL, Floor SN. RNA. 2024 Jul 16;30(8):1041-1057. doi: 10.1261/rna.079837.123. 10.1261/rna.079837.123 PubMed 38697667
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