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Addgene

pEVS104
(Plasmid #207393)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207393 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK2013
  • Backbone manufacturer
    NA
  • Backbone size (bp) 51330
  • Modifications to backbone
    Removed: ColE1 origin of replication, Tn903, and colicin genes. Inserted: R6Kγ origin, second oriT linked to a kanR gene.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid was originally constructed by restriction digestion; original publication: https://doi.org/10.1016/S0076-6879(02)58106-4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEVS104 was a gift from Jason Peters & Edward Ruby & Eric Stabb (Addgene plasmid # 207393 ; http://n2t.net/addgene:207393 ; RRID:Addgene_207393)

  • For your References section:

    Complete sequences of conjugal helper plasmids pRK2013 and pEVS104. Hall A, Donohue T, Peters J. MicroPubl Biol. 2023 Jul 13;2023:10.17912/micropub.biology.000882. doi: 10.17912/micropub.biology.000882. eCollection 2023. 10.17912/micropub.biology.000882 PubMed 37521139
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