pEVS104
(Plasmid
#207393)
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Purpose(Empty Backbone) Conjugal helper plasmid
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207393 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK2013
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Backbone manufacturerNA
- Backbone size (bp) 51330
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Modifications to backboneRemoved: ColE1 origin of replication, Tn903, and colicin genes. Inserted: R6Kγ origin, second oriT linked to a kanR gene.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberLow Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid was originally constructed by restriction digestion; original publication: https://doi.org/10.1016/S0076-6879(02)58106-4
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEVS104 was a gift from Jason Peters & Edward Ruby & Eric Stabb (Addgene plasmid # 207393 ; http://n2t.net/addgene:207393 ; RRID:Addgene_207393) -
For your References section:
Complete sequences of conjugal helper plasmids pRK2013 and pEVS104. Hall A, Donohue T, Peters J. MicroPubl Biol. 2023 Jul 13;2023:10.17912/micropub.biology.000882. doi: 10.17912/micropub.biology.000882. eCollection 2023. 10.17912/micropub.biology.000882 PubMed 37521139
Map uploaded by the depositor.
