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Addgene

pcDNA3 GFP GFP
(Plasmid #20738)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20738 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3-GFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pcDNA3-GFP was created by inserting GFP into the HindIII and BamHI sites of pcDNA3. pcDNA3 GFP-GFP was then prepared by inserting GFP into the HindIII site of pcDNA3-GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 GFP GFP was a gift from Alonzo Ross (Addgene plasmid # 20738)

  • For your References section:

    PTEN enters the nucleus by diffusion. Liu F, Wagner S, Campbell RB, Nickerson JA, Schiffer CA, Ross AH. J Cell Biochem. 2005 Oct 1. 96(2):221-34. 10.1002/jcb.20525 PubMed 16088943
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