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Addgene

pET15b+His SUMO Rab10(Q68L)
(Plasmid #207342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207342 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab10
  • Species
    H. sapiens (human)
  • Mutation
    Q68L
  • Entrez Gene
    RAB10
  • Tag / Fusion Protein
    • His-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b+His SUMO Rab10(Q68L) was a gift from Suzanne Pfeffer (Addgene plasmid # 207342 ; http://n2t.net/addgene:207342 ; RRID:Addgene_207342)

  • For your References section:

    Localization of PPM1H phosphatase tunes Parkinson's disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis. Wondwossen M Yeshaw , Ayan Adhikari, Claire Y Chiang, Herschel S Dhekne, Paulina S Wawro, Suzanne R Pfeffer. Proc Natl Acad Sci U S A. 2023 Oct 31;120(44) 10.1073/pnas.2315171120
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