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Addgene

pHCMV_YFV_PrME
(Plasmid #207315)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 207315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHCMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4764
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFV_PrME
  • Species
    Yellow fever virus
  • Insert Size (bp)
    2037
  • Mutation
    mammalian codon optimization
  • Entrez Gene
    POLY (a.k.a. YFVgp1)
  • Promoter CMV promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCMV_YFV_PrME was a gift from Feng Zhang (Addgene plasmid # 207315 ; http://n2t.net/addgene:207315 ; RRID:Addgene_207315)

  • For your References section:

    Cell type-specific delivery by modular envelope design. Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F. Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8. 10.1038/s41467-023-40788-8 PubMed 37612276
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