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pHCMV_SARS-CoV-2_Sdel18
(Plasmid #207307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207307 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHCMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4764
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV2_S
  • Species
    SARS2 coronavirus
  • Insert Size (bp)
    3812
  • Mutation
    mammalian codon optimization
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCMV_SARS-CoV-2_Sdel18 was a gift from Feng Zhang (Addgene plasmid # 207307 ; http://n2t.net/addgene:207307 ; RRID:Addgene_207307)

  • For your References section:

    Cell type-specific delivery by modular envelope design. Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F. Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8. 10.1038/s41467-023-40788-8 PubMed 37612276
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