pHCMV_RVFV_GP
(Plasmid
#207304)
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PurposeMammalian expression of Rift valley fever virus GP protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
Availability may be limited outside the U.S. Please log in for more information.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207304 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHCMV
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4764
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRVFV_GP
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SpeciesRift valley fever virus
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Insert Size (bp)3591
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Mutationmammalian codon optimization
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Entrez GeneRVFV_sM_gp1 (a.k.a. RVFV_sM_gp1)
- Promoter CMV promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (not destroyed)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bysynthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCMV_RVFV_GP was a gift from Feng Zhang (Addgene plasmid # 207304 ; http://n2t.net/addgene:207304 ; RRID:Addgene_207304) -
For your References section:
Cell type-specific delivery by modular envelope design. Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F. Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8. 10.1038/s41467-023-40788-8 PubMed 37612276
