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Addgene

pHCMV_DENV3_PrME
(Plasmid #207265)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHCMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4764
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DENV3_PrME
  • Species
    Dengue virus 3
  • Insert Size (bp)
    2025
  • Mutation
    mammalian codon optimization
  • Entrez Gene
    POLY (a.k.a. DV3_gp1, polyprotein gene)
  • Promoter CMV promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCMV_DENV3_PrME was a gift from Feng Zhang (Addgene plasmid # 207265 ; http://n2t.net/addgene:207265 ; RRID:Addgene_207265)

  • For your References section:

    Cell type-specific delivery by modular envelope design. Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F. Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8. 10.1038/s41467-023-40788-8 PubMed 37612276
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