iNos-eGFP
(Plasmid #207251)

• Purpose: Reporter for expression of eGFP under control of iNos promoter
• Depositing Lab: Michelle Farkas
• Publication: Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi: 10.3389/fonc.2023.1151384. eCollection 2023.

Backbone

• Vector backbone: pRRLSIN.cPPT.PGK-GFP.WPRE
• Backbone manufacturer: Trono Lab
• Backbone size w/o insert (bp): 7388
• Total vector size (bp): 8669
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: EGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: iNos Promoter
• Alt name: iNos
• Alt name: inducible nitric oxide synthase
• Species: M. musculus (mouse)
• Insert Size (bp): 1825
• Entrez Gene: Nos2 (a.k.a. MAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CCGCTCGAGCGGCGAGCTCTTACGCGGACTTT
• 3′ sequencing primer: CGCGGATCCGCGTTTACCAACAGTACCGGAAT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To generate the iNos-eGFP plasmid, a 1825 -bp XhoI/BamHI fragment was isolated from a ppGL2-NOS2 Promoter-Luciferase construct (Addgene plasmid # 19296, deposited by Charles Lowenste). The iNos fragment was subcloned into the lentiviral construct pRRLSIN.cPPT.PGK-GFP.WPRE (Addgene plasmid #12252, deposited by Didier Trono to generate a PER2-luciferase reporter construct.