Pxyl-rsaA
(Plasmid
#207250)
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PurposeIntegrative plasmid for expressing rsaA gene (incl. its promoter) in Caulobacter crescentus at native Pxyl promoter region
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMCS-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namersaA (promoter to codon sequence)
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SpeciesCaulobacter crescentus
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pxyl-rsaA was a gift from Suliana Manley (Addgene plasmid # 207250 ; http://n2t.net/addgene:207250 ; RRID:Addgene_207250) -
For your References section:
Chromosome organization shapes replisome dynamics in Caulobacter crescentus. Zhang C, Joseph AM, Casini L, Collier J, Badrinarayanan A, Manley S. Nat Commun. 2024 Apr 24;15(1):3460. doi: 10.1038/s41467-024-47849-6. 10.1038/s41467-024-47849-6 PubMed 38658616