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Addgene

pLV-EF1a-CRISPRoffv2.1-IRES-BlastR
(Plasmid #207180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-EF1a-gI-BlastR
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPRoffv2.1
  • Species
    Synthetic
  • Promoter EF1a
  • Tags / Fusion Proteins
    • TagBFP (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Bersten Lab uses DH5alpha cells for transformation. Plasmid can be grown in NEB Stable but this is not required as this Lenti backbone is observed to be very stable.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EF1a-CRISPRoffv2.1-IRES-BlastR was a gift from David Bersten (Addgene plasmid # 207180 ; http://n2t.net/addgene:207180 ; RRID:Addgene_207180)

  • For your References section:

    dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. bioRxiv 2023.11.21.568191 10.1101/2023.11.21.568191
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