pLV-EgI-HygroR
(Plasmid
#207177)
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Purpose(Empty Backbone) Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV
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Backbone manufacturerinhouse
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEmpty
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-EgI-HygroR was a gift from David Bersten (Addgene plasmid # 207177 ; http://n2t.net/addgene:207177 ; RRID:Addgene_207177) -
For your References section:
dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. bioRxiv 2023.11.21.568191 10.1101/2023.11.21.568191