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Addgene

pENTR1a-CRISPRoffv2.1
(Plasmid #207174)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR1a
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPRoffv2.1
  • Species
    Synthetic
  • Tag / Fusion Protein
    • TagBFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR1a-CRISPRoffv2.1 was a gift from David Bersten (Addgene plasmid # 207174 ; http://n2t.net/addgene:207174 ; RRID:Addgene_207174)

  • For your References section:

    dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. bioRxiv 2023.11.21.568191 10.1101/2023.11.21.568191
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