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Addgene

pEF-IRES-puro6 gal4DBD-HIFCAD myc tag
(Plasmid #207171)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207171 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-IRES-Puro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal4DBD-HIF1a-CAD Myc tag linker
  • Species
    H. sapiens (human), Synthetic
  • Promoter EF1a
  • Tag / Fusion Protein
    • 2xMyc tag

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF-IRES-puro6 gal4DBD-HIFCAD myc tag was a gift from David Bersten (Addgene plasmid # 207171 ; http://n2t.net/addgene:207171 ; RRID:Addgene_207171)

  • For your References section:

    dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening. Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC. bioRxiv 2023.11.21.568191 10.1101/2023.11.21.568191
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