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pLV-REPORT (PGK) 5xGRE-NanoLuc
(Plasmid #207170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-REPORT
  • Backbone manufacturer
    inhouse
  • Vector type
    Lentiviral, Luciferase, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    NanoLuciferase
  • Species
    Synthetic
  • Promoter Gal4 response element minP
  • Tag / Fusion Protein
    • PEST (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    d2eGFP
  • Species
    Synthetic
  • Promoter PGK
  • Tag / Fusion Protein
    • 3x SV40 NLS (N terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    5x GRE
  • Alt name
    5x Gal RE
  • Alt name
    5x gal response element
  • Species
    Synthetic

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Bersten Lab uses DH5alpha cells for transformation. Plasmid can be grown in NEB Stable but this is not required as this Lenti backbone is observed to be very stable.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-REPORT (PGK) 5xGRE-NanoLuc was a gift from David Bersten (Addgene plasmid # 207170 ; http://n2t.net/addgene:207170 ; RRID:Addgene_207170)

  • For your References section:

    NanoFIRE: A NanoLuciferase and Fluorescent Integrated Reporter Element for Robust and Sensitive Investigation of HIF and Other Signalling Pathways. Roennfeldt AE, Allen TP, Trowbridge BN, Beard MR, Whitelaw ML, Russell DL, Bersten DC, Peet DJ. Biomolecules. 2023 Oct 19;13(10):1545. doi: 10.3390/biom13101545. 10.3390/biom13101545 PubMed 37892227
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