pCDNA3-myc3-ROC1
(Plasmid #20717)

• Depositing Lab: Yue Xiong
• Publication: He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4620
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RING finger protein 1 (ROC1)
• Alt name: ROC1, RNF75, RBX1
• Alt name: RBX1 ring-box 1
• Alt name: Regulator of Cullins 1
• Species: H. sapiens (human)
• Insert Size (bp): 323
• GenBank ID: AF142059
• Entrez Gene: RBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
• Tag / Fusion Protein:
  • 3xMyc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry