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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20717 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4620
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRING finger protein 1 (ROC1)
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Alt nameROC1, RNF75, RBX1
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Alt nameRBX1 ring-box 1
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Alt nameRegulator of Cullins 1
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SpeciesH. sapiens (human)
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Insert Size (bp)323
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GenBank IDAF142059
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Entrez GeneRBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
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Tag
/ Fusion Protein
- 3xMyc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-myc3-ROC1 was a gift from Yue Xiong (Addgene plasmid # 20717 ; http://n2t.net/addgene:20717 ; RRID:Addgene_20717) -
For your References section:
DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases. He YJ, McCall CM, Hu J, Zeng Y, Xiong Y. Genes Dev. 2006 Nov 1. 20(21):2949-54. 10.1101/gad.1483206 PubMed 17079684