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Addgene

optoAKT
(Plasmid #207164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 3948
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    optoAKT was a gift from Kai Zhang (Addgene plasmid # 207164 ; http://n2t.net/addgene:207164 ; RRID:Addgene_207164)

  • For your References section:

    Optical control of ERK and AKT signaling promotes axon regeneration and functional recovery of PNS and CNS in Drosophila. Wang Q, Fan H, Li F, Skeeters SS, Krishnamurthy VV, Song Y, Zhang K. Elife. 2020 Oct 6;9:e57395. doi: 10.7554/eLife.57395. 10.7554/eLife.57395 PubMed 33021199
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