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pDSK519-cspB
(Plasmid #207162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDSK519
  • Backbone size w/o insert (bp) 10458
  • Total vector size (bp) 10880
  • Modifications to backbone
    chloramphenicol cassette added
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cspB
  • Species
    Clavibacter michiganensis
  • Insert Size (bp)
    420
  • GenBank ID
    WP_012039159.1
  • Promoter nptII
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer GGTGGTcatatgATGCCCACCGGCAAGGTGAAGTT
  • 3′ sequencing primer GGTGGTgaattcTCAGGCATCGAGCTCCTCCGCGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCPPB382 C. michiganensis cspB was codon-optimized for Pseudomonas syringae pv. tomato DC3000.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDSK519-cspB was a gift from Gitta Coaker (Addgene plasmid # 207162 ; http://n2t.net/addgene:207162 ; RRID:Addgene_207162)

  • For your References section:

    Natural variation of immune epitopes reveals intrabacterial antagonism. Stevens DM, Moreno-Perez A, Weisberg AJ, Ramsing C, Fliegmann J, Zhang N, Madrigal M, Martin G, Steinbrenner A, Felix G, Coaker G. Proc Natl Acad Sci U S A. 2024 Jun 4;121(23):e2319499121. doi: 10.1073/pnas.2319499121. Epub 2024 May 30. 10.1073/pnas.2319499121 PubMed 38814867
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