pcDNA3.1(+)Gγ1-LSS-mKATE2(N)
(Plasmid
#207159)
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PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207159 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNG1
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SpeciesH. sapiens (human)
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MutationN-terminally tagged with LSS-mKATE2
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)Gγ1-LSS-mKATE2(N) was a gift from Dominique Schols & Tom Van Loy (Addgene plasmid # 207159 ; http://n2t.net/addgene:207159 ; RRID:Addgene_207159) -
For your References section:
REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors. Boon K, Vanalken N, Meyen E, Schols D, Van Loy T. Biosensors (Basel). 2023 Jul 28;13(8):767. doi: 10.3390/bios13080767. 10.3390/bios13080767 PubMed 37622853