pcDNA3.1(+)GαoB-91-NLuc
(Plasmid
#207152)
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PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNAO
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SpeciesH. sapiens (human)
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MutationNLuc inserted at position L(91)G
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)GαoB-91-NLuc was a gift from Dominique Schols & Tom Van Loy (Addgene plasmid # 207152 ; http://n2t.net/addgene:207152 ; RRID:Addgene_207152) -
For your References section:
REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors. Boon K, Vanalken N, Meyen E, Schols D, Van Loy T. Biosensors (Basel). 2023 Jul 28;13(8):767. doi: 10.3390/bios13080767. 10.3390/bios13080767 PubMed 37622853