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Addgene

pcDNA3.1(+)Gαi1-91-NLuc
(Plasmid #207148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GNAI1
  • Species
    H. sapiens (human)
  • Mutation
    NLuc inserted at position L(91)K
  • Entrez Gene
    GNAI1 (a.k.a. Gi, HG1B, NEDHISB)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)Gαi1-91-NLuc was a gift from Dominique Schols & Tom Van Loy (Addgene plasmid # 207148 ; http://n2t.net/addgene:207148 ; RRID:Addgene_207148)

  • For your References section:

    REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors. Boon K, Vanalken N, Meyen E, Schols D, Van Loy T. Biosensors (Basel). 2023 Jul 28;13(8):767. doi: 10.3390/bios13080767. 10.3390/bios13080767 PubMed 37622853
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