pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR
(Plasmid
#207135)
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PurposeDNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II Ks(+)
- Backbone size w/o insert (bp) 2891
- Total vector size (bp) 5412
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit
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SpeciesM. musculus (mouse)
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Insert Size (bp)2521
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GenBank IDNM_001122733.1
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Entrez GeneKit (a.k.a. Bs, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, c-KIT)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR was a gift from Silvia Monticelli (Addgene plasmid # 207135 ; http://n2t.net/addgene:207135 ; RRID:Addgene_207135) -
For your References section:
The mRNA methyltransferase Mettl3 modulates cytokine mRNA stability and limits functional responses in mast cells. Leoni C, Bataclan M, Ito-Kureha T, Heissmeyer V, Monticelli S. Nat Commun. 2023 Jun 29;14(1):3862. doi: 10.1038/s41467-023-39614-y. 10.1038/s41467-023-39614-y PubMed 37386028