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Addgene

pMMK201
(Plasmid #207131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ptac promoter
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ptac lacO promoter is a hybrid of the lac and trp promoters amplified from the pANT4 plasmid (Lee & Falkow 1998 Infect Immun).

Please visit https://www.biorxiv.org/content/10.1101/2023.05.08.539906v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMMK201 was a gift from Nicholas Shikuma (Addgene plasmid # 207131 ; http://n2t.net/addgene:207131 ; RRID:Addgene_207131)

  • For your References section:

    A modular plasmid toolkit applied in marine bacteria reveals functional insights during bacteria-stimulated metamorphosis. Alker AT, Farrell MV, Aspiras AE, Dunbar TL, Fedoriouk A, Jones JE, Mikhail SR, Salcedo GY, Moore BS, Shikuma NJ. mBio. 2023 Aug 2:e0150223. doi: 10.1128/mbio.01502-23. 10.1128/mbio.01502-23 PubMed 37530556
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