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pcDNA3-HA2-Cul4A-deltaROC
(Plasmid #20713)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA2
  • Backbone size w/o insert (bp) 5512
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cullin 4A
  • Alt name
    CUL4A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2982
  • Mutation
    Deletion of residues 594 to 612 from N-terminus
  • GenBank ID
    NM_001008895
  • Entrez Gene
    CUL4A
  • Tag / Fusion Protein
    • HA2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Data not available (not destroyed)
  • 3′ cloning site Data not available (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

K644R mutation is present but does not affect function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA2-Cul4A-deltaROC was a gift from Yue Xiong (Addgene plasmid # 20713 ; http://n2t.net/addgene:20713 ; RRID:Addgene_20713)

  • For your References section:

    Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development. McCall CM, Miliani de Marval PL, Chastain PD, Jackson SC, He YJ, Kotake Y, Cook JG, Xiong Y. Mol Cell Biol. 2008 Sep . 28(18):5621-33. 10.1128/MCB.00232-08 PubMed 18606781
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