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pSCALPS-FLAG-HA-mmuMettl3 APPA
(Plasmid #207124)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207124 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSCALPS
  • Backbone size w/o insert (bp) 7740
  • Total vector size (bp) 9559
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mettl3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1765
  • Mutation
    D395A and W398A
  • GenBank ID
    NM_019721.2
  • Entrez Gene
    Mettl3 (a.k.a. 2310024F18Rik, M6A, Spo8)
  • Promoter SSFV
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GCCACCATGGACTACAAGGA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCALPS-FLAG-HA-mmuMettl3 APPA was a gift from Silvia Monticelli (Addgene plasmid # 207124 ; http://n2t.net/addgene:207124 ; RRID:Addgene_207124)

  • For your References section:

    The mRNA methyltransferase Mettl3 modulates cytokine mRNA stability and limits functional responses in mast cells. Leoni C, Bataclan M, Ito-Kureha T, Heissmeyer V, Monticelli S. Nat Commun. 2023 Jun 29;14(1):3862. doi: 10.1038/s41467-023-39614-y. 10.1038/s41467-023-39614-y PubMed 37386028
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