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Addgene

pGEX Itk TEC
(Plasmid #20711)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Itk TEC
  • Alt name
    IL2-inducible T-cell kinase
  • Species
    M. musculus (mouse)
  • Mutation
    amino acids 97-174
  • Entrez Gene
    Itk (a.k.a. Emt, Tcsk, Tsk)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX Itk TEC was a gift from Leslie Berg (Addgene plasmid # 20711 ; http://n2t.net/addgene:20711 ; RRID:Addgene_20711)

  • For your References section:

    Biochemical interactions integrating Itk with the T cell receptor-initiated signaling cascade. Bunnell SC, Diehn M, Yaffe MB, Findell PR, Cantley LC, Berg LJ. J Biol Chem. 2000 Jan 21. 275(3):2219-30. 10.1074/jbc.275.3.2219 PubMed 10636929
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