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Addgene

pEF Itk W208K myc
(Plasmid #20710)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Itk SH3*
  • Alt name
    IL2-inducible T-cell kinase
  • Species
    M. musculus (mouse)
  • Mutation
    W208K. Inactivates binding pocket of SH3 domain.
  • Entrez Gene
    Itk (a.k.a. Emt, Tcsk, Tsk)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

W208K. See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF Itk W208K myc was a gift from Leslie Berg (Addgene plasmid # 20710 ; http://n2t.net/addgene:20710 ; RRID:Addgene_20710)

  • For your References section:

    Biochemical interactions integrating Itk with the T cell receptor-initiated signaling cascade. Bunnell SC, Diehn M, Yaffe MB, Findell PR, Cantley LC, Berg LJ. J Biol Chem. 2000 Jan 21. 275(3):2219-30. 10.1074/jbc.275.3.2219 PubMed 10636929
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