pEF Itk P158A P159A myc
(Plasmid
#20708)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF-BOS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameItk PR
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Alt nameIL2-inducible T-cell kinase
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SpeciesM. musculus (mouse)
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MutationP158A P159A. Inactivates the proline-rich region.
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Entrez GeneItk (a.k.a. Emt, Tcsk, Tsk)
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Tag
/ Fusion Protein
- myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF-1a forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
P158A P159A (adds a BspEI site). See author's map for more information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF Itk P158A P159A myc was a gift from Leslie Berg (Addgene plasmid # 20708 ; http://n2t.net/addgene:20708 ; RRID:Addgene_20708) -
For your References section:
Biochemical interactions integrating Itk with the T cell receptor-initiated signaling cascade. Bunnell SC, Diehn M, Yaffe MB, Findell PR, Cantley LC, Berg LJ. J Biol Chem. 2000 Jan 21. 275(3):2219-30. 10.1074/jbc.275.3.2219 PubMed 10636929
Map uploaded by the depositor.
