ClhN-p1k-Vrg4-V5-APEX2-URA
(Plasmid
#207071)
-
PurposeExpressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneClhN-URA
-
Vector typeYeast Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameVrg4
-
SpeciesS. cerevisiae (budding yeast)
- Promoter pVRG4
-
Tag
/ Fusion Protein
- V5-APEX2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ClhN-p1k-Vrg4-V5-APEX2-URA was a gift from Zhiping Xie (Addgene plasmid # 207071 ; http://n2t.net/addgene:207071 ; RRID:Addgene_207071) -
For your References section:
A Validated Set of Ascorbate Peroxidase-Based Organelle Markers for Electron Microscopy of Saccharomyces cerevisiae. Li H, He CW, Zhu J, Xie Z. mSphere. 2022 Aug 31;7(4):e0010722. doi: 10.1128/msphere.00107-22. Epub 2022 Jun 21. 10.1128/msphere.00107-22 PubMed 35727034
