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Addgene

ClhN-pADH3-Tgl3-4V5-APEX2-URA
(Plasmid #207066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ClhN-URA
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tgl3
  • Species
    S. cerevisiae (budding yeast)
  • Promoter pADH3
  • Tag / Fusion Protein
    • 4xV5-APEX2 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ClhN-pADH3-Tgl3-4V5-APEX2-URA was a gift from Zhiping Xie (Addgene plasmid # 207066 ; http://n2t.net/addgene:207066 ; RRID:Addgene_207066)

  • For your References section:

    A Validated Set of Ascorbate Peroxidase-Based Organelle Markers for Electron Microscopy of Saccharomyces cerevisiae. Li H, He CW, Zhu J, Xie Z. mSphere. 2022 Aug 31;7(4):e0010722. doi: 10.1128/msphere.00107-22. Epub 2022 Jun 21. 10.1128/msphere.00107-22 PubMed 35727034
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