pEF Itk myc
(Plasmid #20705)

• Depositing Lab: Leslie Berg
• Publication: Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30.

Backbone

• Vector backbone: pEF-BOS
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Itk
• Alt name: IL2-inducible T-cell kinase
• Species: M. musculus (mouse)
• Entrez Gene: Itk (a.k.a. Emt, Tcsk, Tsk)
• Tag / Fusion Protein:
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EF-1a forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Wild-type Itk. See author's map for more information. The native stop codon of Itk was deleted and replaced with a PCR-derived fragment encoding a short linker peptide and a carboxyl-terminal Myc epitope tag. These modifications extend Itk by 21 amino acids (AGAEFMEQKLISEEDLRKFDI). EF/Itk-myc expression vectors were generated by inserting the modified Itk cDNA into a derivative of pEF-BOS.