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Addgene

P1K-HFL1-8flag-LEU
(Plasmid #207035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNH605
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HFL1
  • Species
    S. cerevisiae (budding yeast)
  • Promoter pHFL1
  • Tag / Fusion Protein
    • 8xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P1K-HFL1-8flag-LEU was a gift from Zhiping Xie (Addgene plasmid # 207035 ; http://n2t.net/addgene:207035 ; RRID:Addgene_207035)

  • For your References section:

    Membrane recruitment of Atg8 by Hfl1 facilitates turnover of vacuolar membrane proteins in yeast cells approaching stationary phase. He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, Gong Q, Xie Z. BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7. 10.1186/s12915-021-01048-7 PubMed 34088313
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