Skip to main content
Addgene

BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
(Plasmid #207024)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207024 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    BS-ZJLEU
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HFL1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    D384A Y387A
  • Promoter pTEF1
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP was a gift from Zhiping Xie (Addgene plasmid # 207024 ; http://n2t.net/addgene:207024 ; RRID:Addgene_207024)

  • For your References section:

    Membrane recruitment of Atg8 by Hfl1 facilitates turnover of vacuolar membrane proteins in yeast cells approaching stationary phase. He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, Gong Q, Xie Z. BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7. 10.1186/s12915-021-01048-7 PubMed 34088313
Related items: