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Addgene

pHM830
(Plasmid #20702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5352
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-MCS-LacZ
  • Species
    Artificial
  • Insert Size (bp)
    4015

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence uploaded to AddGene might not be 100% correct - if necessary verify critical areas by sequencing

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHM830 was a gift from Thomas Stamminger (Addgene plasmid # 20702 ; http://n2t.net/addgene:20702 ; RRID:Addgene_20702)

  • For your References section:

    Mapping of nuclear localization signals by simultaneous fusion to green fluorescent protein and to beta-galactosidase. Sorg G, Stamminger T. Biotechniques. 1999 May . 26(5):858-62. 10.2144/99265bm12 PubMed 10337476
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