BS-ZJLEU-PTEF1-GFP
(Plasmid
#207018)
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PurposeOver-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 207018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneBS-ZJLEU
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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SpeciesS. cerevisiae (budding yeast)
- Promoter pTEF1
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BS-ZJLEU-PTEF1-GFP was a gift from Zhiping Xie (Addgene plasmid # 207018 ; http://n2t.net/addgene:207018 ; RRID:Addgene_207018) -
For your References section:
Membrane recruitment of Atg8 by Hfl1 facilitates turnover of vacuolar membrane proteins in yeast cells approaching stationary phase. He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, Gong Q, Xie Z. BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7. 10.1186/s12915-021-01048-7 PubMed 34088313